Rat anti human IL-2:PE Bio-Rad MCA1553PE IL-2抗体-产品中心-瑞孚迪Revvity和PerkinElmer珀金埃尔默官网，revvity瑞孚迪 (Revvity)为生命科学和诊断业务公司

Rat anti human IL-2:PE Bio-Rad MCA1553PE IL-2抗体

2024-10-22

Rat anti human IL-2:PE

货号：MCA1553PE

规格：100 Tests/1ml

市场价格：3188

产品类型：流式抗体

品牌：Bio-Rad

抗原： IL-2

物种：人

宿主：大鼠

抗体亚型：IgG2a

克隆号：MQ1-17H12

荧光染料：PE

产品介绍
Rat anti Human Interleukin-2 antibody, clone MQ1-17H12recognizes human interleukin-2 (IL-2), also known as T-cell growth factor or Aldesleukin. IL-2 is a 153 amino acid secreted cytokine produced by T-cells following antigenic or mitogenic stimulation. It plays a crucial role in T-cell proliferation and immune regulation.Rat anti Human Interleukin-2 antibody, clone MQ1-17H12 has been sucessfully used for the detection and measurement of native and recombinant human IL-2 using flow cytometry and ELISA where it ascts as the capture reagent in a very sensitive assay for IL-2 determination in human samples. IL-2 is used extensively for the treatment of patients with metastatic melanoma (Atkinset al.1999) and renal cancer with reproducible benificial effects (Rosenberg 2014). Monitoring of IL-2 levels in such patients is required for optimization of treatment regimens (Lotzeet al.1986). Rat anti Human Interleukin-2 antibody, clone MQ1-17H12 is also useful for evaluating levels of IL-2 in patients with autoimmune conditions including lupus erythematosus and rheumatoid arthritis where there may be an impaired ability to produce IL-2 (Talalet al.1983).
产品详情
Target Species	Human
Species Cross-Reactivity	Target Species	Cross Reactivity
Cynomolgus monkey	√
N.B. Antibody reactivity and working conditions may vary between species.
Product Form	Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution	Reconstitute with 1.0ml distilled water
Preparation	Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
BufferSolution	Phosphate buffered saline
Preservative Stabilisers	0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 5% Sucrose
Max Ex/Em	Fluorophore	Excitation Max (nm)	Emission Max (nm)
RPE 488nm laser	496	578
Regulatory	For research purposes only
Guarantee	12 monthsfrom date of despatch
存储条件
Prior to reconstitution store at +4oC.After reconstitution store at +4oC.DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
应用
Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry1	√		Neat
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.¹Membrane permeabilization is required for this application. Bio-Rad recommend the use of Leucoperm™(Product CodeBUF09) for this purpose.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Flow CytometryUse 10ul of the suggested working dilution to label 1x10⁶cells in 100ul.
同型
Description	Product Code	Applications	Pack Size
Rat IgG2a Negative Control:RPE	MCA6005PE	Flow	100 Tests
相关产品
Description	Product Code	Applications	Pack Size
Human Seroblock	BUF070A	Flow	50 Tests
Human Seroblock	BUF070B	Flow	200 Tests
数据
Figure A. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A) for 5 hours were stained with Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and PE conjugated Rat IgG2a (MCA6005PE). Figure B. Cells stimulated with Cell Stimulation Reagent containing Brefeldin A (BUF077A)for 5 hours were stained with Alexa Fluor 488 conjugated Mouse anti Human CD3 (MCA463A488) and PE conjugated Mouse anti Human IL-2 (MCA1553PE). All experiments performed on red cell lysed human blood gated on lymphoid cells in the presence of 10% human serum. Data acquired on the ZE5™ Cell Analyzer
文献
1. Garbe, Y.et al.(2011) Semiallogenic fusions of MSI(+) tumor cells and activated B cells induce MSI-specific T cell responses.BMC Cancer. 11: 410.2. Moncrieffe, H.et al.(2010) High expression of the ectonucleotidase CD39 on T cells from the inflamed site identifies two distinct populations, one regulatory and one memory T cell population.J Immunol. 185 (1): 134-43.

技术参数

产品应用 Flow Cytometry

产品类型 Monoclonal Antibody

推荐用于 Flow Cytometry

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